Abstract: Objective: To investigate the association between Survivin expression and helicobacter pylori (HP) infec-tion in gastrointestinal B cell Lymphoma (BCL). Methods: Using immunohistochemistry, we detected the expression ofSurvivin genes in tissues from 56 cases of gastrointestinal BCL (30 cases of gastric BCL and 26 cases of intestinal BCL)and 30 normal gastrointestinal mucosal tissue samples. HP was detected by histological fuchsine staining. Results: Sur-vivin expression was found in 14 of the 30 cases of gastric BCL (46.67%) and in 11 of the 26 cases of intestine BCL(30.77%). There was not significant difference between the two groups (P>0.05). The total expression rate of Survivin was44.64%(25/56) in gastrointestinal BCL group and 0% in the control group (P<0.01). The expression rate of Survivin was20.83%(5/24) in low malignant gastrointestinal BCL and 65.63%(21/32) in high malignant gastrointestinal BCL, with a sig-nificant difference (P<0.01). The infective rate of HP was 46.67%(14/30) in gastric BCL and 30.77%(8/26) in intestinalBCL, with no significant difference(P>0.05). The infective rate of HP was 39.29%(22/56) in the gastrointestinal BCL groupand 13.33%(4/30) in the normal group, with a statistical significance (P<0.05). The HP positive rate was 66.67%(16/24) inmucosa-associated lymphoid tissue(MALT) lymphoma and 18.75%(6/32) in non-MALT lymphoma(P<0.01). The expressionof Survivin in gastrointestinal BCL was related to HP infection. Conclusion: The expression of Survivin involves in the de-velopment of gastrointestinal BCL and is related to HP infection.