Abstract: Objective: : To explore the influence of carbon dioxide pneumoperitoneum-laparoscopic surgery on tumorcell seeding and metastases in early-stage cervical cancer. Methods : From September 2005 to September2006, 20 patients with cervical cancer who underwent laparoscopic surgery and 10 patients with cervical cancer who underwent laparotomic surgery were selected. Each patient was FIGO stage Ia2-IIa. Carbon dioxidepneumoperitoneum was established and maintained with CO₂ insufflation at 4-6L/min and intraperitoneal pressure of 13 mmHg with an automatic pneumoperitoneum machine. Cytologic examination of peritoneal fluid (atthe beginning and end of operation), CO₂Results: There were no cases of positive washing cytology in peritoneal fluid, CO₂P>0.05). The difference in abnormal expression of E-cadherin and β-catenin protein between the two groups were not statistically significant (P>0.05). The protein expression of MMP-2, P-selectin, VEGF and CD44v6 were not significantly different between the pre-and post-operative samples in laparoscopic group and laparotomic group (P>0.05). The fol-low-up period was 15-27 (24.85±3.53) months in the laparoscopic group and 15-27 (24.20±4.47) months inthe laparotomy group. No recurrence was observed in laparoscopic group. One case of recurrence was seenin laparotomy group. Conclusion : Laparoscopic surgery for cervical cancer has no effect on protein expressionof E-cadherin, β-catenin, P-selectin, MMP-2, VEGF, and CD44v6 in tumor tissues. There is no evidence thatCO₂ pneumoperitoneum-laparoscopic surgery may promote seeding and metastasis of early cervical cancer.