Abstract: Objective : To screen for protein markers from serum of patients with pancreatic carcinoma by SELDI-TOF-MS. Methodse : A case-control study of 18 serum samples from patients with pancteatic carcinoma and 18 serum samples from healthy control was carried out with strong anionic exchange proteinchip. Results : Analyzed by SELDI-TOF-MS with SAX, eight protein biomarkers were identified including three up-regulated protein biomarkers with relative molecular weights of 4136.60, 4465.92 and 4359.53Da, respectively and five down-regulated ones with relative molecular weights of 5856.8, 7564.52, 5116.1, 2042.78 and 2016.44 Da, respectively in the serum of patients with pancreatic carcinoma compared with the healthy controls. There was a statistical difference in one protein biomarker with weight of 4136.60Da between 11 cases of pancreatic duct adenocarcinoma and 7 cases of other kinds of carcinoma. ROC curve analysis revealed that these six identified protein biomarkers had a sensitivity of 83%-94% and a specificity of 56%-67% for the diagnosis of pancreatic carcinoma. Conclusion : Some potential protein biomarkers with good sensitivity and specificity in the serum of patients with pancreatic carcinoma can be helpful for the diagnosis of pancreatic carcinoma.