Abstract: Objective : To evaluate the effect of Dipeptidyl Peptidase Ⅳ on the invasion and metastasis of prostatic carcinoma cell line PC-3M-1E8. Methodse : Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to amplify dipeptidyl peptidase Ⅳ (DDPⅣ) cDNA fragments. RT-PCR products were cloned into plasmid pEGFP-C1 in the proper orientation for expressing antisense RNA, and the recombinant pEGFP-C1/DDPⅣ was transfected into 1E8 cells. We employed the CCK-8 assay to detect changes in cell proliferation. DDPⅣ protein expression and invasiveness were detected by Western blot and wound healing assay, respectively. Results : The eukaryotic expression vector pEGFP-C1/DDPⅣ designed to produce antisense DDPⅣ RNA was constructed successfully. At 36 hours and 48 hours after transfection with pEGFP-C1/DDPⅣ, the proliferation of 1E8 cells was significantly higher than that of untransfected cells. Compared with control cells, the pEGFP-C1/DDPⅣ-transfected cells displayed decreased expression of endogenous DDPⅣ protein. Tumor cell motility and invasive potential were enhanced in pEGFP-C1/DDPⅣ-transfected cells. Conclusion : DDPⅣ is a tumor suppressor gene that inhibits metastasis of prostatic cancer. Proliferation and invasive potential of 1E8 cells were enhanced by antisense DDPIV, suggesting that DDPⅣ may be useful in gene therapy for prostatic carcinoma.