Abstract: Objective: To detect the expression of B-myb and C-myc in hepatocellular carcinoma(HCC) tissues andto explore its significance in the occurrence, progression and metastasis of HCC. Methods: Immunohistochemisty was em-ployed to detect the expression of B-myb and C-myc in 60 samples of HCC tissues and their adjacent liver tissues. Results: The positive rates of B-myb and C-myc in the HCC tissues were higher than in the adjacent liver tissues (56.67%and 53.33% vs. 36.67% and 35%, P<0.05). The expression of B-myb in the HCC tissues was significantly correlated withthe clinical stage, the presence of extrahepatic metastasis, the recurrence of tumor, the number of tumors, and capsule oftumor. The expression of C-myc in the HCC tissues was significantly correlated with portal vein tumor thrombus, the pres-ence of extrahepatic metastasis, the recurrence of tumor, the differentiation and capsule of tumor. The expression of B-myb was positively correlated with the expression of C-myc in HCC tissues. Conclusion: The over-expression of B-myband C-myc proteins may contribute to the proliferation of hepatocellular carcinoma cells and is related to the occurrenceand progression of HCC.