Abstract: Objective: To evaluate the role of hypoxia-inducible factor-1α (HIF-1α) and angiogenic factors in peri-toneal metastases and prognosis of advanced serous ovarian cancer. Methods: Sections of effusions and primary andmetastatic lesions from 31 ovarian serous carcinoma patients were evaluated for the expression of hypoxia-inducible fac-tor-1α (HIF-1α), basic-broblast factor(bFGF), and vascular endothelial growth factor(VEGF) by immunohistochemistry (I-HC). Results: HIF-1αand VEGF expression showed a remarkable downregulation in the effusions, compared with thosein the primary and metastatic sites (P<0.05). In contrast, constantly high expression of bFGF was observed in all of thethree kinds of samples. The size of the residual mass, the expression of HIF-1α, VEGF, bFGF and MVD in tumor cellswere correlated with cancer recurrence (P<0.05). In a multivariate Cox regression model, VEGF and MVD were indepen-dent prognostic factors (P<0.05). Conclusion: HIF-1α, VEGF and bFGF play important roles in peritoneal metastasis ofserous ovarian cancer. VEGF and MVD were independent prognostic factors for patients with advanced serous ovarian cancer.