CDA-2对人子宫内膜癌细胞增殖及分化的影响

贾晋萍; 钟雪云; 林琛莅

CDA-2对人子宫内膜癌细胞增殖及分化的影响

The Effect of Uroacitide on the Proliferation and Differentiation of Human Endometrial Carcinoma Cell Line EAC

摘要

摘要: 目的: 研究尿多酸肽(CDA-2)对EAC人子宫内膜癌细胞增殖、分化及细胞自分泌的影响。 方法: 应用MTT法、集落形成实验检测CDA-2对EAC细胞增殖的影响;通过光镜观察和免疫组化鉴定细胞分化;放射免疫法检测细胞自分泌雌二醇(E₂)、孕酮(P)。 结果: CDA-2使EAC细胞增殖与集落形成均明显受到抑制,并呈剂量依赖性。CDA-2处理72h后:EAC出现细胞分化的形态学改变,免疫组化显示ER、PR表达阴性。EAC细胞无自分泌E₂、P。 结论: CDA-2不仅能抑制子宫内膜癌EAC细胞增殖,而且能诱导EAC细胞分化。

Abstract: Objective: To study the effect of Uroacitide (CDA-2) on the proliferation, differentiation and autocrine ofhuman endometrial carcinoma cell line EAC. Methods: MTT assay and colony formation assay were employed to detectthe effect of CDA-2 on cellular survival and proliferation. The differentiation of EAC cell line was identified by HE stain-ing and immunocytochemistry. E2 and P in endometrial carcinoma cell line EAC were quantified by radioimmunoassay. Results: The cellular survival and proliferation of EAC cell line were remarkably inhibited by CDA-2 and the effect wasdose-dependent. After being treated with CDA-2 for 72 hours, HE staining of EAC cells revealed differentiation inducedby CDA-2. No expression of ER and PR were observed by immunocytochemistry. No E2 and P were detected in EACcells. Conclusion: CDA-2 could inhibit proliferation and induce differentiation in human endometrial carcinoma cell lineEAC.

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