食管癌线粒体DNA微卫星不稳与核DNA微卫星不稳相关性探讨

The Relationgship between Mitochondrial Microsatellite DNA Instability and Nuclear Microsatellite DNA Instability in Esophageal Cancer

摘要: 目的:探讨食管癌线粒体DNA微卫星不稳与核DNA微卫星不稳的关系。方法:采用PCR单链构象多态性(PCR-SSCP)方法检测食管癌及正常组织细胞mtMSI与nMSI。结果:45例食管癌检出mtMSI9例(20.0%),检出nMSI10例(22.2%)。mtMSI主要发生于D-loop区。mtMSI与nMSI两者的发生有相关性(P<0.05)。结论:食管癌组织中存在mtMSI,mtMSI的发生可能通过nMSI参与了食管癌的发生过程。

Abstract: Objective : To evaluate the relationship between mitochondrial microsatellite DNA instability and nuclei microsatellite DNA instability in esophageal cancer. Methods : mtMSI and nMSI were analyzed by PC-SSCP methods. Results : The mtMSI was detected in 9 of 45 (20.0%). The ntMSI was detected in 11 of 45 (24.42%). mtMSI was mainly detected in mitochondrial DNAD-loop.The association was observed between mtMSI phenotype and nMSI phenotype (P<0.05). Conclusion :The mtMSI was found in the esophageal cancer tissue, and mtMSI may participate in the carcinogenesis of the esophageal cancer through nMSI.