Abstract:
Objective : The analysis on differential gene expression profiles of human lung carcinoma cell lines with different metstatic phenotype.
Methods : To prepare the probes,mRNA from cells were isolated and purified, then reversely transcribed to cDNA with the incorporation of fluorecentla-beled dUTP. The probes were hybridized with a cDNA microarray. The chip were scanned by ScanAr-ray 4000 laser scanner on two wavelengths. The acquired image was analyzed by Gene Pix Pro 3.0 soft-ware. The intensity of each spot on the two wavelengths presented the quantity of Cy3-dUTP and Cy5-dUTP, with Cy5 to Cy3 ratio computed on each.
Results : 20genes exhibited differential expressions in 2 lung carcinoma cell lines. 6genes were down-regulated, 14 gene were up-regulated.
Conclusion : cDNA microarry provides an promising approach to specific gene expressions of the presence of metas-tasis in human lung carcinoma.