Abstract: Objective : To study the expression of Matrix Metalloproteinase (MMP)2, MMP9 and Ki-67 in human gliomas and the relationship between tumor proliferation and invasion. Methods : Tu-mor invasion was examed as the expression level of (MMP) 2 and MMP9. Proliferative activity of glioma was evaluated by Ki-67 Labling Index (Ki-67 LI) , and Immunohistochemical study was used for Ki-67, MMP2 and MMP9 expression in six normal brain tissue samples, fifty glioma samples and two glioblastoma cell lines. Results : The positive stained-cell rate of MMP2 and MMP9 increased with the degree of malignancy of tumors, and their differences of positive rate and expression level between the low-grade and high-grade tumors were also statistically significant. Ki-67 LI also increased corre-spondingly to the ascending of tumor grade, and the Ki-67 LI in grade III and IV was higher than that in low-grade gliomas. In addition, the expression of MMP2, MMP9 and Ki-67 correlated well with each other by Person correlation analysis. Conclusion : Proliferation and invasion cooperate with each other to promote the development of malignant phenotypes of gliomas.