Abstract: Objective ： To explore the morphologic and immunohistochemical features of gastroin-testinal stromal tumors (GISTs) and distribution features of pathologically benign, potential malignancy, malignancy. Methods ： To select J5 cases which had complete clinical records and correct pathologic diagnosis, we use the immunohistochemical staining for CD117, CD34, SMA, S-100, NSE to all cases. Results : In 35 cases of GISTs, malignant (include potential malignancy) occupy 85T over.23 cases were spindle cell type (65.7%), 7 cases were epithelioid cell type (20%) and 5 cases mixed cell type (14.2%). Immunohistochemical positive staining showed: CD117 74.3% (26/35cases), CD34 68.6% (24/35 cases), NSE 65.7% (23/35 cases), S-100p 31.4% (11/35 cases), SM A 48.6% (17/35 cases). Neural and smooth muscle differentiation were observed in 13 and 5 cases. Both smooth muscle and neural differentiation were 12 cases. No neural or smooth muscle differentiation were 5 cases. Conclusions ： GISTs were the most frequent mesenchymal tumor seen in the gastrointestinal tract. The application oi immunohistochemical markers CD117 and CD34 are mutually benefit for a final correct diagnosis.