Abstract: Objective: To evaluate the clinical efficacy of cisplatin (CDDP), Leucovorin (LY) plus fluorouracil (5-FU) therapy in esophageal carcinoma and randomly compare the efficacy and toxicity of 5-FU intravenous injection with continuous intravenous infusion in this regimen. Methods: One hundred and twenty-two patients with advanced esophageal carcinoma objectively evaluated and diagnosed by histopathology, were randomly divided into 5-FU intravenous injection group (A group) and continuous intravenous infusion group (B group). They all have firstly received two to four cycles of CDDP-LY/ 5-FU therapy. Results: The overall response rate (CR+PR) was 69.67%(85/122), and the response rate of A group was 76.47%(39/51), which was higher than that of B group 64.79%(46/71). The difference between the two groups had statistical significance (P<0.05); the toxicity of A group was higher than that of B group (P<0.05), however, most of them had toxicity of grade " or 瓜,and all were tolerable and reversible. Conclusions: CDDP-LY/5-FU therapy had higher response rate in esophageal carcinoma. The efficacy and toxicity of 5-FU intravenous injection were higher than those of continuous intravenous infusion. It can be concluded that both 5-FU intravenous injection and continuous intravenous infusion in this regimen have reliable response rate in esophageal carcinoma and the intravenous injection is comparatively better.