Abstract: Objective :To observe the inhibiting effect of antisense oligodeoxyribonueleotide (A-SODN) on Survivin gene expression in osteosarcoma cells. Methods :To synthesize the ASODN of specific target Survivin with different concentrations and transfect them on osteosarcoma OS-732 cell lines;at the same time,set up the blank control group and sense oligodeoxyribonucleotide (SODN) group for comparison. Observe the condition of cell growth before and after transfection; adopt RT-PCR,Western blot and immunocytochemical technique to detect Survivin mRNA and protein expression of cells in each group.The further treated cells of each group were inoculated into nude mice, and then the condition of tumorigenesis was observed. Results :Comparing with control and SODN groups, the cell's quantity was decreased and their growth was delayed in each transfection ASODN group;the expression of Survivin mRNA and protein were decreased,and the effect mentioned above exhibited a dose-dependent manner;tumorigenesis in the vivo of nude mice was delayed, and the weight of tumor was significantly lower than that of control and SODN group. Conclusion :ASODN can block the survivin gene expression in OS-732 cells specificallythe suppress tumor cell growth and the viability.