结肠癌细胞转染IL-21基因抑瘤效应的实验研究

The Antitumor Research of Murine Colon Carcinoma Cells Transduced With The Mouse IL-21 Gene

摘要: 目的:初步探讨转染IL-21的结肠癌细胞抑制肿瘤生长的效应。方法:以逆转录病毒为载体将IL-21基因导入结肠癌细胞,筛选建立高表达细胞株,测定其生物学性状指标和抑制肿瘤生长的效果。结果:与原株相比,所建立的Colon26/IL-21细胞株可高质量的表达IL-21,在体内可刺激分泌大量的IFNγ,致使结肠肿瘤明显回缩。结论:IL-21是一种高效的抗肿瘤细胞因子,有望成为结肠癌基因治疗的的候选基因。

Abstract: Objective:To investigate the antitumor responses of inhibiting the colon cells growth by interleukin-21(IL-21) gene tranferredinto into murine colon carcinoma cells. Methods: We transduced murine colon carcinoma cells with the mouse IL-21 gene and select the high secretion cell strain, observe the effection of inhibiting the growth of colon tumor. Result: Compared with the control cell group, the transfeced Colon26/IL-21 cell strain could express IL-21 moleecular and induce the large amounts of IFNy secreted, which result in the Colon tumor evidently shrink. Conclusion: IL-21 is a novel antitumor cytokine, may be selected as a waitful gene in gene therapy of colon carcinomao