Abstract: Objective:To further improve the reversed effect, we attempted to take the Tetramethylpyrazine (TMP) in combination withp-elemene which were different in effect mechanism, andreversed the MDR (multidrug resistnce) of K562/ADM via more links. Methods: The sensitivity of cell lines and the reversal of resistance were determined with MTT method. The cell apoptosis was determined by flow cytometry and transmission electron microscope. Statistical treatment with SPSS was applied to deal with all the experimental data. Results: 1, The non-cytotoxic dose of TMP (350N.,g/ml)and(3-elemene (4.ON.,g/ml) could markedly decrease the ICSO value of K562/ADM cell line to adriamycin(ADM) and increase the sensitivity of K562/ADM cell line to ADM. The resistance一二versing effect was 2.03-fold and 2.18-fold respectively. The non一cytotoxic dose of (3-elemene (4.ON.,g/ml) could induce apoptosis effectively; 2, The non一〔eytotoxic dose of TMP combined with (3-elemene strongly improved drug resistance reversal effect (4.65-fold), clearly, which was larger than the single application of TMP(350N.,g/ml) orp-elemene(4.ON.,g/ml) respectively and their summation. We also found these two medicines were helpful to increase the intracellular ADM concentration and to induce cell apoptosis.Conclusion: Administration of TMP in combination with p-elemene has obvious co-effect on the resistance-reversing and the inducement of apoptosis in K562/ADM cell line.