Abstract: Objective:To investigate the expression of survivin and its relationship with the ex-pression of PTEN and the cyclinDl. Methods: By flow cytometry (FCM) and immunofluorescence, the expression of survivin, PTEN and cyclinDl were determined in 48 cases of hepatocellular carcinoma,32 cases of paraneoplastic cirrhosis, 6 cases of cirrhosis, 6 cases of normal hepatic tissue. Results: The expressional level of survivin and cyclin D1 in tumor tissues were obviously higher than that in the paraneoplastic tissue, cirrhosis and normal hepatic tissue, but the expressional level of PTEN was much lower. The expression of survivin and PTEN was significantly correlated with the histological differentianon and prognosis, but not with the tumor size, the presence of portal vein thrombosis and the intrahepatic metastasis. With the progress of malignancy, significant correlation was observed between the survivin and the cyclinDl, while negative correlation was observed between the survivin and PTEN ex- pression in hepatocellular carcinoma. Conclusion: This experiment sugguests that the survivin, the PTEN and the cyclin D 1 may play different roles in the oncogenesis and progression of hepatocellular carcinoma. Joint detection of the survivin and PTEN gene products can supply certain evidence on prognosis of hepatocellular carcinoma.