Abstract: Objective :The loss of genetic material from specific chromosome loci is a common feature in the tumorigenesis and is often indicative of the presence of important tumor suppressor genes(TSG) at these loci. Loss of heterozygosity(LOH) is one of the important procedure to detect TSG. The aim of this study is to identify additional loci involved in sporadic colorectal carcinoma(CRC). Methods : D10S1265, a fluorescent labeled polymorphic microsatellite marker, was analyzed in 83 cases of sporadic CRC and normal tissue DNA by PCR. PCR products were eletrophoresed on an ABI 377 DNA sequencer. Genescan 3.1 and Genotype 2.1 software were used for LOH scanning and analysis. Comparison between LOH frequency and clinicopathological factors were performed by χ² test. Results : The LOH frequency of D10S1265 locus (10q24.3) was 50.0%. On this locus, there was no LOH accompanied within liver metastasis patients(0/7), while 58.97%(23/39) without liver metastasis (P=0.014). Additional significant correlation was detected between LOH frequency and Dukes stages (P=0.013). Conclusion : This study seems to show the evidence of the TSG on 10q24.3 related to the sporadic CRC, which may be involved in the progression and metastasis.