食管癌患者微卫星杂合性缺失特征及其意义

The Character and Significance in Loss of Heterozygosity of the Patients from High-incidence Area of Esophageal Carcinoma by Microsatellite Analysis

摘要: 目的 :探讨与食管癌发病密切相关的微卫星杂合缺失(lossofheterozygosity,LOH)变化特征及其意义。方法 :将32例原发性食管癌手术切除标本采用微卫星DNA多态分析法,选取染色体3p、5q、6p、9p、13q、16q、17p、17q和18q的18个多态微卫星位点,对食管癌组织进行LOH分析。 结果: 18个微卫星位点均获得有效数据。 结论: 抑癌基因RASSF1A、APC、p15、BRCA2、p53、BRCA1和DCC的LOH可能是食管癌发生过程中重要的分子事件。

Abstract: Objective : To discuss the characters and changes of microsatellite LOH closely related to esophageal cancer (EC) and its significance in esophageal carcinogenesis. Methods : Microsatellite polymorphism analysis was performed for allelic deletions with 18 polymorphic markers at the site of chromosome 3p, 5q, 6p, 9p, 13q, 16q, 17, and 18q on surgically resected EC specimens and matched normal esophageal tissues, which were collected from Linzhou people's Hospital and Linzhou Central Hospital, the high incidence area for EC in Henan. Results : Valid data were obtained at the 18 microsatellite sites. Conclusion : LOH of RASSF1A, APC, p15, BRCA2, p53, BRCA1 and DCC may be the important molecular events involved in the esophageal carcinogenesis in Henan, the high-incidence area for EC.