整合素受体介导bcl-2反义核酸对人结肠癌细胞的靶向作用

Targeting Effect of bcl-2 Antisense Oligodeoxynucleotide Mediated by Integrin Receptor on Human Colon Carcinoma Cell Line caco-2

摘要: 目的 :用聚乙烯亚胺-整合素配体复合物(PEI-RGD)介导的bcl-2反义核酸作用于结肠癌细胞caco-2,观察其对细胞的靶向作用。方法 :用流式细胞仪测定细胞对荧光标记的反义核酸的摄取率和平均荧光强度,并在相差/光显微镜下观察摄入情况。 结果: 使用PEI-RGD介导的反义核酸与单独使用反义核酸的caco-2细胞摄入率和荧平均荧光强度存在明显差异(P<0.05)。与正常肝细胞LO2相比较,用PEI-RGD介导法显著提高结肠癌细胞caco-2对bcl-2反义核酸的摄入,且有统计学意义(P<0.05)。 结论: 阳离子复合物PEI-RGD可以增加caco-2对bcl-2反义核酸的摄入,具有靶向作用。

Abstract: Objective : To investigate the targeting effect of bcl-2 ASODN mediated by PEI-RGD on human colon carcinoma cell line caco-2. Methods : The mean intracellular fluorescence intensity of cells and uptake rates of fluorescence-labled bcl-2 antisense oligodeoxynucleotide were measured by flow cytometry, and were observed with phase-contrast fluorescence microscopy. Results : Compared with the control group, the uptake rate of bcl-2 antisense oligodeoxynucleotide into caco-2 cell and the mean intracellular fluorescence intensity of cells were greatly improved by PEI-RGD (P<0.05). Conclusion : PEI-RGD can efficiently enhance the cellular of uptake rates of bcl-2 antisense oligodeoxynucleotide in caco-2 cells, which has targeting effect.