Abstract: Objective :To prepare a new tumor vaccine and observe its anti-umor effects. Methods :The vaccine was prepared by fusion of human red blood cells and rabbit VX2 tumor cells using PEG. MTT tests were used to measure its ability to activate cytotoxic lymphocytes (CTL) in vitro.Its in vivo effect on tumor recurrence and metastasis was also evaluated using animal model. Results :The vaccine had a remarkable effect on the proliferation of peripheral blood mononuclear cells. The mononuclear cells after priming exhibited an obvious and specific cytotoxicity towards VX2 cells in vitro. In vivo, the vaccine could remarkably reduce the rate of VX2 tumor recurrence and metastasis. Conclusions :The vaccine could induce specific anti-umor effects both in vitro and in viva. Our results provide useful data for further study on human tumor fusion vaccine.