Abstract: Objective: To detect the expression of centromere protein F (CENP- F) in brain glioma and to study its clinical significance. M ethods: Immunohistochemistry was used to detect the expression of CENP- F in 64 cases of brain glioma and 10 cases of normal brain tissues by paraffin- embedded. Results: The positive percentage of CENP-F expression in 64 cases with brain glioma and 10 normal branin tissues was 76.6% and 30.0%, respectively. There was a significant difference between brain glioma and normal brain tissues (P<0.01). For the expression of CENP- f in brain glioma, there was no significant difference among sex, age, tumor site, tumor size, peritumoral edema and pathological types (P>0.05), but a significant difference among pathological grades (P<0.05). Conclusion: CENP- F levels are high in brain glioma and are concerned with pathological grades, which may play a role in judgement of the biologic behavior of brain glioma.