人肺癌中NF-κBDNA结合活性的意义探讨

The Significance of Nuclear Factor-KappaB DNA Binding Activity in Human Lung Cancer

摘要: 目的:观察在肺癌组织中核因子-κB(Nuclearfactor-kappaB,NF-κB)的DNA结合活性,探讨其在肺癌发展中的意义。方法:采用电泳迁移率(Electrophoretic mobility shift assay,EMSA)同位素放射自显影的方法分别检测有随访资料的87例肺癌组织中NF-κB的DNA结合活性。结果:NF-κBDNA结合活性在小细胞肺癌组织中高于其它病理类型,在低分化肺癌组织中结合活性较高,在淋巴结转移肺癌组织中结合活性高于无淋巴结转移肺癌组织(P<0.05)。结论:NF-κB的DNA结合活性与肺癌的病理类型、分化程度及是否有淋巴结转移等因素有关。

Abstract: Objective: To investigate the nuclear factor- kappaB (NF-κB) DNA binding activity in lung cancer tissue and its significance in development of lung cancer. Methods: The NF-κB DNA binding activity of 87 cases with lung cancer tissue was detected using electrophoretic mobility shift assay (EMSA). Results: The NF-κB DNA binding activity was higher in small cell lung cancer tissue,compared to cancer with other pathological types; the binding activity was higher in the tissue of poorly- differentiated cancer, and was higher in the lung cancer tissues with lymph node metastasis than with non lymph node metastasis (P<0.05). Conclusions: The NF-κB DNA binding activity is correlated with factors, such as histological types, degree of differentiation and lymph node metastasis.