王晓燕, 马春红, 张艳, 高立芬, 朱法良, 郭春, 刘华. 脂质体介导EndostatinIL-12基因联合治疗移植型鼠肝癌的实验研究[J]. 中国肿瘤临床, 2005, 32(16): 951-954.
引用本文: 王晓燕, 马春红, 张艳, 高立芬, 朱法良, 郭春, 刘华. 脂质体介导EndostatinIL-12基因联合治疗移植型鼠肝癌的实验研究[J]. 中国肿瘤临床, 2005, 32(16): 951-954.
shu
Wang Xiao-yan, Ma Chun-hong, Zhang Yan, . Experimental Study on Endostatin and Interleukin-12 Genes Mediated by Lipofectine in Treatment of Transplanted Liver Cancer in Mice[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2005, 32(16): 951-954.
Citation: Wang Xiao-yan, Ma Chun-hong, Zhang Yan, . Experimental Study on Endostatin and Interleukin-12 Genes Mediated by Lipofectine in Treatment of Transplanted Liver Cancer in Mice[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2005, 32(16): 951-954.
shu

脂质体介导EndostatinIL-12基因联合治疗移植型鼠肝癌的实验研究

Experimental Study on Endostatin and Interleukin-12 Genes Mediated by Lipofectine in Treatment of Transplanted Liver Cancer in Mice

    摘要
  • 摘要: 目的:探讨脂质体介导Endostatin及IL-12基因联合治疗肝癌的可行性。方法:将已构建的Endostatin和IL-12真核表达载体给荷瘤BALB/c鼠瘤内注射,每周两次测量瘤体积。ELISA法检测肿瘤局部IL-12、IL-2、Endostatin含量,MTT法检测NK细胞杀伤活性,流式细胞仪检测肿瘤细胞凋亡情况。结果:联合治疗组肿瘤体积明显小于其它各组(P<0.05);肿瘤局部IL-12、IL-2、Endostatin的表达明显高于pVAX1空载体和生理盐水对照组(P<0.05);NK细胞杀伤率和肿瘤细胞凋亡率分别为52.0%和48.2%。结论:联合应用Endostatin和IL-12基因可有效抑制鼠肝癌的生长。

     

    Abstract: Objective: To investigate the feasibility of endostatin and interleukin- 12 genes mediated by lipofectine in treatment of liver cancer. Methods: Recombinant endostatin and IL- 12 eukaryotic expression vectors were used to inject intratumorly BALB/c mice bearing with hepatocarcinoma cells.The size of tumors was messured two times every week. The concentration of IL- 12, IL- 2, endostatin in situ of tumor was detected by ELISA, the cytotoxity activity of NK cells was detected by MTT. Apoptosis of tumor cells were detected by Flow Cytometry. Results: The size of tumors in combined treatment group was significantly smaller than that in other groups (P<0.05). The expression of IL- 12 、 IL- 2 、 endostatin in combined treatment group were significantly higher than that in pVAX1 vector and normal saline control group (P<0.05). Cytotoxity ratio of NK cells and apoptosis of tumor cells in combined treatment group was respectively 52.0% and 48.2%. Conclusion: Combined therapy of endostatin and interleukin- 12 gene can effectively inhibit the growth of murine liver cancer.

     

    • HTML全文
    • 参考文献(1)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回