Abstract: Objective : To explore the relationship between the basement membrane (BM) and extracellular matrix (ECM) and the infiltration and metastasis of lung cancer and to explore the mechanism of Chinese herb elemene in anticancer function. Methods : Lewis lung cancer model with C57BL/6 inbreeding line mice was set up. Forty mice were divided into the normal group, model group, CTX group and the Elemene group. The hyaluronic acid (HA) and collagen Ⅳ(colⅣ) in blood serum and the laminin (LN) and fibronectin (FN) in transplatation tumour tissue were measured by the method of radioimmunology and of SABC immunohistochemistry, respectively. Results : In Elemene group, contents of HA and CollY in blood serum were decreased, lineal shape expression of FN on BM and ECM around tumour cells was maintained, and LN in tumour cells was decreased, which were all significantly different from those in both the model group and the CTX group. Conclusion : The action mechanisms of Chinese herb Elemene are different from those of chemical CTX. The partial action mechanisms of Chinese herb Elemene on inhibiting lung cells metastasis are to protect BM and ECM barrier and to decrease matrix component expression in tumour cells.