Abstract:
Objective :To clone the full length of gastric cancer related gene expressed segment,analyze its biological characters and to predict protein function with bioinformatics.
Methods :Differendally expressed cDNA fragments wire obtained through fluorescent differential display PCR in gastriccancer and its pre- malignant lesions and normal gastric mucosa tissues. Cloned full length cDNA of the sequence I which no homologous sequences in databases with 5RACE, and identified the reality of sequences using Northern blot method. The databases of NCBI and EMBL was used to analyze its biolcal characters of the coded amino acid ORF and to predict the function of encoded polypeptide.
Results :The full- length cDNA of seqence I was obtained using 5RACE method. The ORF sequence wasnamed as gcl, with two trans-membrane sections. Results of function sites searched by PROSITE revealed that gcI had three protein kinase C phosphorylation sites and three Nmyristoylation sites.
Conclusions :gel, a novel gastric cancer related gene encoding a trans-menbrane protein related to signaltransduction, might be a putative gene concerned with gastric cancer genesis.