Abstract: Neoadjuvant chemotherapy is a systemic chemotherapy before surgical operation or radiotherapy. It is good for the breast cancer with the recent response rate and it offers the credible evidence to susceptibility test of chemotherapeutic agent in vivo. The evaluation on the response rate of neoadjuvant chemotherapy is only limited to the routine method of pathological histomorphology below the microscope. It is a new essay to analyze the relationship between the tumor markers and chemotherapy reaction, in order to observe the change of breast cancer tissues in different aspects after neoadjuvant chemotherapy with better prospect of clinical individual chemotherapy.