Abstract: Objective: To investigate the apoptosis induced by Psoralean, and the relationship between intracellular Ca²⁺ Concentration and apoptosis. Methods: MTT assay, Annexin V FITC and laser scanning confocal microscopy were used to study the inhibitory effect of HL60 cells on cell proliferation and the induction of apoptosis ratio in HL60 cells. Results: Psoralean can inhibit the growth of HL60 cells, increasing the ratio of apoptosis, Ca²⁺Concentration and apoptosis is positive correlation. Conclusion: Psoralean can influence HL60 cells apoptosis and the Ca²⁺-induced apoptosis is one of the possible mechanisms of Psoralean inhibiting the growth of cancer cell.