Abstract: Objective :To investigate the effects of integrin-β₁ antisense oligodexynucleotides(ASODNs)on cell-growth and chemosensitivity of pancreatic cancinoma cell line BXPC-3. Methods :Integrin-β₁ antisense oligodexynucleotides was transfected into BXPC-3 with liposome.The expression of integrin-β₁ mRNA was determined by RT-PCR.The viability rate was assessed by MTT method.Different concentrations of 5-FU,adriamycin(ADM),Gemcitabine were given 24 h after cells transfected with integrin-β₁ ASODNs.The dose-effects responses were observed and IC₅₀ s were calculated. Results :Transfecting BXPC-3 cells with 32mg/L～128mg/L integrin-β₁ ASODNs inhibited growth significantly and reduced expression of integrin-β₁ mRNA.Integrin-β₁ ASODNs increased the chemosensitivity of BXPC-3 cells to 5-FU,ADM,Gemcitabine.The IC₅₀ s of different chemotherapy groups were significantly lower than those in controls. Conclusion :Integrin-β₁ ASODNs can effectively decrease the expression of integrin-β₁ gene,inhibits cell growth and enhances the chemosensitivity in pancreatic cancinoma cell line BXPC-3.