Abstract: Objective: To investigate the changes of gene expression profile in pancreatic adenocarcinoma cell line SW1990 after triptolide treatment, in order to find the possible triptolide targets. Methods: Total RNA of triptolide-treated or untreated SW1990 cells was isolated and used as templates for reverse transcriptional labeling of Cy5 and Cy3 fluorescent cDNA probes, respectively. High density DNA microarray chips with a set of 4096 human genes/Ests were used to generate the expression profile by hybridizing with fluorescently labeled probes. Array image was screened and analyzed with array analyzing software GenePixPro3.0. To confirm the expression profiles of these genes, two of the down-regulated genes, C-MYC and ETS2 were selected to undergo analysis by real-time PCR. Results: Incubated with triptolide for 4 h, 11 genes expression in SW1990 cells were significantly suppressed. Conclusion: Potential down-regulated targets from this study include C-MYC, ETS2, TGIF and RTP801, as well as the others. It is suggested that the widely known anti-tumor effects of triptolide are mediated at least in part by suppression of these gene expression.