Abstract: Objective : To study the practical value of multiple sites biopsy in lymph nodes of endometrial carcinoma. Methods : One hundred and sixty-five patients with endometrial carcinoma, who accepted the multiple sites biopsy in retroperitoneal lymph nodes after undergoing primary surgical treatments in our hospital from January 1990 to December 2000, were analyzed retrospectively. The metastasis rates of retroperitoneal lymph nodes with different clinical pathologic characteristics, the overall 5-year survival rates with or without lymph nodes metastasis, as well as with different numbers of positive lymph nodes among the patients, were compared. Results : The enlarged lymph nodes were palpated in forty of the one hundred and sixty-five patients. There was a significant difference between sixteen patients with metastasis about 76.19% (16/21) and twenty four patients with no metastasis account for 16.55% (24/144) (P<0.001). The patients who positive lymph nodes without enlarged take up 4.00% (5/125). in 21 patients with positive retroperitoneal lymph nodes, The 5-year survival rates was 23.81%, the median survival was 30.2 months and in 165 patients with negative lymph (who were followed up more than 5 years). The overall 5-year survival rate was 66.36%, the median survival was 77.3 months. The survival rates between patients with positive and negative lymph nodes compared showed significant difference (P<0.001). Ten patients possessed less than or equal to three of positive lymph nodes, their overall 5-year survival rates was 50.00%, the median survival were 42.1 months,while 11 patients possessed more than or equal to four of positive lymph nodes, no 5-year survival, the median survival were 21.3 months, comparing two group with different numbers of positive lymph nodes show significant difference (P<0.001). bv univariate analysis, clinical stages, histological types, patho-logical grades, depth of myometrial invasions, cervical invasions, parametrial and appendix invasions as well as lymphovascular invasions related to retroperitoneal lymph nodes positive (P<0.05), those eight factors were significantly correlated to lymph nodes positive multivariate analysis (P<0.05). Conclusion :The surgical-pathological staging is more accurate in multiple sites of retroperitoneal lymph nodes biopsy which is one of important prognostic factors, the retroperitoneal lymph nodes in multiple sites biopsy can be completed by i t routine in stages I and II for endometrial carcinoma.