Abstract: Objective: To explore the bone healing and revascularization process and the mechanism of microwave-induced devitalized bone by animal experiment. Methods: The tibia segments of NewZealand rabbits were devitalized by microwave during operation and then re-implanted. The specimens were taken for examination of X-ray, electron microscope scan, fluorescence microscope and SPECT bone scan at 3, 6, 12 and 24 weeks points postoperatively. The union time, change of fluorescence, absorption of radioactive 99mTc-MDP and formation of lamellar bone were studied and self contra-lateral control was conducted. Results: Devitalized bone healed at 6 week point and shaped mostly at 24 week point. Tetracycline fluorescence firstly occurred in the surrounding of Haversian Canal and continued to expand. The dense of radioactivity began to reduce gradually from the bottom of bone segments to the middle during the knitting. Conclusion: The microwave-induced devitalized bone could union and the union time and revascularization was slower than the one of fresh autograft. Induce activities and blood supply originating from the bottom were one of the major ways to revascularize for microwave-induced devitalized bone.