Abstract: Objective : To investigate the relationship between the telomerase activity and expres-sion of CD44 v5/v6 and the malignancy and metastasis potency of ovarian cancer. Methods : TRAP-ELISA was used to detect telomerase activity, and expression of CD44 v5/v6 and DNA content was an-alyzed by FCM (Flow cytometry). Results : a) Telomerase activity increased significantly along with clin-ical stage (according to FIGO stage) and pathologic grade (P<0.05 or P<0.01 respectively). It was sig-nificantly higher in patients with lymph node metastasis compared to those without the metastasis, andwas significantly higher in patients with DNA aneuploid compared to those with DNA diploid (P<0.05or P<0.01 respectively). b) The expression of CD44 v5/v6 increased significantly along with clinicalstage and pathologic grade (P<0.05 or P<0.01 respectively). It was significantly higher in patients withlymph node metastasis or DNA aneuploid compared to those without metastasis (P<0.01). c) The expres-sion of CD44 v5/v6 increased significantly along with telomerase activity (P<0.01). Conclusion : Boththe telomerase related with cell proliferation and CD44 v5/v6 in relation with tumor metastasis closelycorrelate with the malignancy and metastasis potency of ovarian cancer, and there is a close correlationbetween them. The malignant behavior of ovarian cancer through different aspects can be evaluated us-ing combined detection.