ω-3脂肪酸对人胃癌细胞凋亡信号传导的影响

The Effect of Omega 3 Fatty Acids on Signal Transduction for Apoptosis of the Human Gastric Cells

摘要: 目的 :观察EPA和DHA对人胃癌BGC-823细胞凋亡信号传导的影响。方法 :采用MTT法测定肿瘤细胞抑制率,流式细胞检测肿瘤细胞凋亡率和bcl-2、Gα、PKCβⅡ基因蛋白的表达。结果 :45μg/ml和48h时间点的EPA或DHA可显著抑制肿瘤细胞的生长(P<0.001),凋亡率明显升高(P<0.001),bcl-2基因蛋白表达下降(P<0.05),Gα和PKCβⅡ基因蛋白表达明显下降(P<0.001)。结论 :ω-3脂肪酸可诱导人胃癌BGC-823细胞发生凋亡,信号传导途径Gα-PKCβⅡ的改变在细胞凋亡中起到重要的作用。

Abstract: Objective : To assess the effects of EPA and DHA on signal transduction for apoptosisof the human gastric BGC-823 cells in vitro. Methods : Inhibitive rate of the cells was determined byMTT assay. Apoptotic rate and expression of bcl-2, Gαand PKCβ Ⅱ proteins were determined usingflow cytometry. Results : After treatment with 45μ g/ml of EPA or DHA and at the exposed point of 48h,the growth of cells was markedly inhibited (P<0.001), and apoptotic rate was markedly increased (P<0.001). Expression of bcl-2 was decreased (P<0.05), and expression of Gαand PKCβ Ⅱ protein weremarkedly decreased (P<0.001). Conclusions : The ω-3 fatty acids can induce apoptosis of human gas-tric BGC-823 cells. Gαand PKCβ Ⅱ signaling may play an important role in apoptosis of the cells.