刘民锋, 余险峰, 郭伟, 左石, 罗剑, 徐立宁, 邹声泉. LY294002对胆管癌细胞化疗增敏作用的探讨[J]. 中国肿瘤临床, 2006, 33(11): 611-613.
引用本文: 刘民锋, 余险峰, 郭伟, 左石, 罗剑, 徐立宁, 邹声泉. LY294002对胆管癌细胞化疗增敏作用的探讨[J]. 中国肿瘤临床, 2006, 33(11): 611-613.
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Liu Minfeng, Yu Xianfeng, Guo Wei, Zuo Shi, Luo Jian, Xu Lining, Zhou Shengquan. LY294002 Sensitizes Cholangiocarcinoma Cells to Chemotherapy[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2006, 33(11): 611-613.
Citation: Liu Minfeng, Yu Xianfeng, Guo Wei, Zuo Shi, Luo Jian, Xu Lining, Zhou Shengquan. LY294002 Sensitizes Cholangiocarcinoma Cells to Chemotherapy[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2006, 33(11): 611-613.
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LY294002对胆管癌细胞化疗增敏作用的探讨

LY294002 Sensitizes Cholangiocarcinoma Cells to Chemotherapy

    摘要
  • 摘要: 目的 :运用磷脂酰肌醇3-激酶抑制剂(phosphatidylinositol3-kinase,PI3-K)LY2940022-(4-吗啉基)-8-苯基-4氢-1-苯并吡喃-4-酮作用于胆管癌细胞系QBC939,探讨抑制PI3K/AKT信号转导通路对胆管癌化疗的增敏作用。 方法 :MTT法检测单独使用5-FU,MMC,celecoxib,联合磷酸化抑制剂LY294002,体外对胆管癌细胞系QBC939的抑制作用;运用流式细胞技术检测QBC939凋亡抑制率。 结果 :运用LY294002后能明显增加5-FU,MMC,celecoxib对胆管癌细胞系QBC939体外培养细胞的抑制作用,并且能提高其凋亡率。 结论 :LY294002能有效提高化疗药物5-FU,MMC,celecoxib体外对QBC939细胞抑制作用的敏感性;抑制PI3K介导的信号转导通路对胆管癌肿瘤的治疗中可能有一定的协同作用。

     

    Abstract: Objective :To explore the specific inhibitor LY294002 of the signaling pathway PI3K/AKT that increases sensitivity to the regular chemotherapeutic agent of cholangiocarcinoma. Methods :The inhibition of cholangiocarcinoma was detected when treated individually with 5-FU, MMC,or cele-coxib, and was compared to a combination of those drugs individually with LY294002.Under the sameconditions,the apoptosis ratio was detected using flow cytometry. Results :The phosphorylation in-hibitor LY294002 can increase sensitivity to 5- FU and MMC and also increase the apoptosis ratio invitro. Conclusion :The phosphorylation inhibitor LY294002 can significantly enhance sensitivity to thechemotherapeutic agent. Inhibition of the PI3K/AKT signaling pathway could have a synergistic effecton treatment of cholangiocarcinoma.

     

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