Abstract: Objective :To study the serum protein fingerprint of patients with gastric cancer duringonset and progression of the disease and to screen for protein molecules closely related to the pathologicstaging of gastric cancer. Methods :Proteins from gastric cancer patients, non-cancer patients, andhealthy blood-donors were screened using the surface-enhanced laser desorption ionization mass spec-trometry (SELDI/TOF-MS) and protein-chip technology, coupled with a pattern-matching algorithm. Results :Some proteins were detected that may be representative for early-stage gastric cancer, andfour of them were 6193, 6363, 16073 and 1631 (M/Z).Some may be related to the metastasis of thegastric cancers, such as 32463 and 34906 (M/Z). Conclusion :The protein-chip technology in conjunc-tion with bioinformatics can screen the significantly different proteins in the serum of gastric cancerpatients with the potential value of further investigation.