Abstract: Objective: To explore the effect of VEGF-C gene transfection on the expression levelof VEGF-C protein in human gastric cancer cell OCUM-2MD3. Methods: The VEGF-C cDNA se-quence, which was obtained from clone vector pCRⅡ-VEGF-C doubly digested with EcoRΙ and XhoΙ,was ligated into an eukaryotic expression vector pcDNA3.1 to construction the recombinant plasmidpcDNA3.1-VEGF-C. The pcDNA3.1-VEGF-C was transformed into human gastric cancer cell OCUM-2MD3 induced by liposome. The changes in the expression level of VEGF-C mRNA and protein were determined by RT-PCR, immunohistochemistry and flow cytometry (FCM). Results: After the digestion,the recombinant plasmid pcDNA3.1-VEGF-C was confirmed to contain the human VEGF-C cDNA se-quence. After transfection, the VEGF-C mRNA level (0.38±0.11)%, the positive rate of VEGF-C pro-tein expression in transformed group (52.16± 6.72)% by immunohistochemistry, and (41.83± 3.65)% by FCM were all dramatically higher than those of the control group (P<0.05 or 0.01), respectively. Con-clusion: After VEGF-C gene transfection by liposome, the expression level of VEGF-C in human gas-tric cancer cell OCUM-2MD3 is greatly heightened. This may be helpful for exploring the mechanismof lymphatic metastasis and its gene therapy in gastric carcinoma.