Abstract: Objective: To apply NS-398 for medicinal intervention of the cell lines of liver cancerand to investigate the influence of NS-398 on apoptosis of hepatocellular carcinoma. Methods: The media containing various concentrations of NS-398 (0, 25, 50, 75 and 100μmol/L) were used for con-centration-dependent experiments. Three time-points (24h, 48h, 72h) of action time were taken foreach concentration group, and the cells were collected daily for 3 days. The inhibition effect of cellgrowth was measured using MTT assay. Changes of cell cycle, the apoptotic cells and expression of sur-vivin, cyclinD1 and PTEN after treatment with NS-398 were detected by flow cytometry method. Re-sults: Treatment of with NS-398 significantly suppressed cell proliferation in time-and dose-depen-dent manner both. The percentage of cells in G₀ /G₁ phase decreased as the percentage of cells in G₂ /Mphase significantly increased in cultures treated with NS-398. NS-398 also induced the apoptosis de-tected by flow cytometry method. We found that NS-398 treatment significantly downregulated the ex-pression of survivin, cyclinD1 and PTEN in a dose- dependence and time-dependent manner (P<0.001).Conclusion: This study suggests that NS-398 can inhibited the proliferation and induced apoptosis of hepatoma cell, which may be partially fulfilled by downregulation of the survivin and PTEN.