Abstract: Objective :To clone a functional human vascular endothelial growth factor C (VEGF-C) cDNA from the MDA-MB231 cell line and to construct a eukaryotic expression vector with this geneto use for further study of the role of the VEGF- C gene in lymphatic dissemination of cervical cancer atthe gene level. Methods :Based on the human VEGF-C cDNA sequence, two pairs of specific primerswere designed and constructed which contained an EcoRI digestion site in the first primer at the 5' endand an XhoⅠ digestion site in the second primer at the 3'end. Reverse transcription polymerase chainreaction (RT-PCR) was employed to clone VEGF-C cDNA from the human MDA-MB231 cell line. Theproduct of RT-PCR was digested with EcoRⅠ, SphⅠ and XhoⅠ. After purifying the product, it wasligated into the eukaryotic expression vector pcDNA3.1(+) that had been digested with EcoRⅠ and XhoⅠ. The recombinant plasmid pcDNA3.1 (+) was first propagated in Escherichia coli DH5α , and then itwas extracted and purified. The recombinant plasmid was confirmed with PCR and digestion with EcoRⅠ and XhoⅠ in addition to DNA sequence analysis. Results :Human VEGF-C cDNA was amplifiedfrom MDA-MB231. The recombinant pcDNA3.1 (+)/VEGF-C vector contained correct nucleotide se-quence for the full length human VEGF-C cDNA fragment by PCR, restriction digestion and DNA se-quence analysis. Conclusion :A eukaryotic expression vector containing full length human VEGF-C inpcDNA3.1 (+) has been successfully constructed.