Abstract:
Objective :To investigate the molecular mechanisms underlying proliferation and apop-tosis in NB4 acute promyelocytic leukemia (APL) cells in culture.
Methods :APL cell line NB4 wasused for in vitro studies. The effect of bufalin on NB4 was studied using morphological analysis, elec-tron microscopy, and RT-PCR.
Results :Bufalin inhibited NB4 cell proliferation in a time-and dose-dependent manner, induced NB4 cell apoptosis and downregulated survivin expression in NB4 cells.
Conclusion :Bufalin can induce apoptosis in NB4 cells through the downregulation of survivin expres-sion.