Abstract: Objective : To evaluate the feasibility for application of the sentinel lymph-node (SLN) biopsy in diagnosis and treatment of gastric cancer. Methods : Thirty-two patients with gastric cancer were divided into three groups (T1, T2, T3 and T4) based on extent of the tumorous invasion. SLN was localized using the method of patent-blue injection. Metastatic lymph nodes were detected by immunohistochemical staining of cytokeratin (CK). Results : The SLNs were detected in all 32 patients and the metastases of lymph nodes occurred in 22 cases, among which the metastasis of SLN were found in 20. The sensitivity, false-negative rate, and diagnostic accuracy of SLN for prediction of the lymph-node metastasis was 91%(20/22), 9%(2/22) and 93.8%(30/32), respectively. The diagnostic accuracy was 100% in the group with T1 cases, without false-negative cases, 100% in T2 cases without false-negative case, 90% in T3 with one false-negative case and 75% in T4 with one false-negative case. Conclusion : Application of the injection of patent blue around tumor is a feasible method for intra-operative localization of SLN.