Abstract: Objective: To determine the adhesion between 5 different types of human tumor cell lines and 4 microvascular endothelia of various sourcesand to analyze the possible correlation between the adhesive capacity and organ-specific metastasis. Methods: After formation of the completely confluent and quiescent endothelial monolayer through microvascualr endothelial culture, the adhesion determination was conducted as the tumor cells were labeled with Calcein AM. The tumor cells attached to endothelium were quantified after incubation and rinsing. Results: Of the 8 esophageal carcinoma cell lines, adhesion between 6 cell lines and human pulmonary endothelium was significantly higher than that of the endothelium of sinus hepaticus, and adhesion between 7 cell lines and normal human esophagus as well as endothelium of the esophageal carcinoma was higher than that of the endothelium of normal human lung and of sinus hepaticus. This was in accordance with clinical characteristics of the esophagus, i.e., pulmonary metastasis was more than liver metastasis and the invasive and disseminated metastasis in the esophagus was much more common compared to distant metastasis, etc. The adhesion between 4 colon carcinoma cell lines and human sinus hepaticus endothelium was significantly higher than that of the endothelium of human lung and of the normal esophagus, which was with according to clinical characteristics of colon carcinoma with liver metastasis. The adhesion between the cell lines of the carcinomas, such as liver cancer, gastric carcinoma and lung cancer, and endothelium was in accordance with the characters of clinical metastases. Conclusion: The adhesive capacity of the human endothelial cells with the tumor cells of different types and organs of various sources has a clear tissue specificity and selectivity and the adhesiveness is closely in relation with clinical tissue specificity of the tumor. To block up the adhesive attraction may be helpful in treating the tumor metastasis.