陈勇军, 唐启彬, 王剑明, 邹声泉. 肝外胆管癌染色体3p21.3区段微卫星不稳定和杂合性缺失分析[J]. 中国肿瘤临床, 2006, 33(20): 1146-1149.
引用本文: 陈勇军, 唐启彬, 王剑明, 邹声泉. 肝外胆管癌染色体3p21.3区段微卫星不稳定和杂合性缺失分析[J]. 中国肿瘤临床, 2006, 33(20): 1146-1149.
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Chen Yongjun, Tang Qibin, Wang Jianming, et al. The Analysis of Microsatellite Instability and Loss of Heterozygosity at Chromosome 3p21.3 in Extrahepatic Cholangiocarcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2006, 33(20): 1146-1149.
Citation: Chen Yongjun, Tang Qibin, Wang Jianming, et al. The Analysis of Microsatellite Instability and Loss of Heterozygosity at Chromosome 3p21.3 in Extrahepatic Cholangiocarcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2006, 33(20): 1146-1149.
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肝外胆管癌染色体3p21.3区段微卫星不稳定和杂合性缺失分析

The Analysis of Microsatellite Instability and Loss of Heterozygosity at Chromosome 3p21.3 in Extrahepatic Cholangiocarcinoma

    摘要
  • 摘要: 目的:研究肝外胆管癌染色体3p21.3区段的微卫星不稳定性(MSI)及杂合性缺失(LOH),探讨染色体3p21.3区段遗传不稳定性与肝外胆管癌的发生发展的关系,定位该区段上肝外胆管癌相关肿瘤基因。方法:用PCR-SSCP方法检测24例肝外胆管癌染色体3p21.3区段上D3S1568,D3S1621,D3S1578和D3S1289四个微卫星位点的MSI和LOH发生率,分析其与临床病理因素之间的关系。结果:24例肝外胆管癌组织中,四个微卫星位点的MSI和LOH平均发生率分别为7.23%和15.63%。其中D3S1621位点的LOH最高(45.83%,11/24),并与TNM分期、是否伴有局部/淋巴结转移相关(p<0.05)。结论:染色体3p21.3区段D3S1621位点高频率杂合性缺失,提示3p21.3区段可能定位有肝外胆管癌的候选抑癌基因,并在肝外胆管癌的发生发展过程中发挥重要作用。

     

    Abstract: Objective: To study the microsatellite instability and loss of heterozygosity (LOH) of the chromosome 3p21.3 segment and to discuss the correlation between genetic instability of the chromosome 3p21.3 segment and morbidity and progression of extrahepatic cholangiocarcinoma. Methods: The incidence of MSI and LOH of the 4 microsatellite loci, i.e., D3S1568, D3S1621, D3S1578 and D3S1289 located at chromosome 3p21.3 in 24 cases with extrahepatic cholangiocarcinoma samples were detected using PCR-SSCP. The relationship between clinical pathology factors and chromosome 3p21.3 instability was analyzed statistically. Results: In extrahepatic cholangiocarcinoma samples of 24 cases, the average incidence rata of MSI and LOH of the 4 microsatellite loci was 7.23% and 15.63%, respectively, among which the highest frequency of LOH occurred at D3S1621(45.83%,11/24) and related significantly to the TNM stage and local or lymphatic metastasis(P<0.05). Conclusion: The high frequency LOH of D3S1621 suggests that there might be some candidate anti-oncogene locating at chromosome 3p21.3 which plays an important role in carcinogenesis and progression of the extrahepatic choloangiocarcinoma.

     

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