蒲丹, 李为民, 陈敏, 陈文彬. HnRNP A2/B1短发夹环RNA重组体的构建与序列分析[J]. 中国肿瘤临床, 2006, 33(24): 1386-1389.
引用本文: 蒲丹, 李为民, 陈敏, 陈文彬. HnRNP A2/B1短发夹环RNA重组体的构建与序列分析[J]. 中国肿瘤临床, 2006, 33(24): 1386-1389.
shu
Pu Dan, Li Weimin, Chen Min, Chen Wenbin. Construction and Sequence Verification of an Expression Vector for shRNA Specific for HnRNP A2/B1[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2006, 33(24): 1386-1389.
Citation: Pu Dan, Li Weimin, Chen Min, Chen Wenbin. Construction and Sequence Verification of an Expression Vector for shRNA Specific for HnRNP A2/B1[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2006, 33(24): 1386-1389.
shu

HnRNP A2/B1短发夹环RNA重组体的构建与序列分析

Construction and Sequence Verification of an Expression Vector for shRNA Specific for HnRNP A2/B1

    摘要
  • 摘要: 目的:利用RNA干扰(RNAinterfering,RNAi)技术构建HnRNPA2/B1重组质粒,并行序列分析。方法:分别将21bP长短的HnRNPA2/B1靶序列,中间为7bP序列间隔的反向重复序列,置入PsiRNA-hH1neoG2质粒中,产生重组质粒PSiRNA-hH1neoG2HnRNPA2/B1。结果:将重组质粒作测序分析,经测序鉴定确定为所需序列。结论:特异性短发夹环RNA(ShRNA)真核表达载体PSiRNA-hH1neoG2HnRNPA2/B1的成功构建,为进一步研究其基因功能奠定基础。

     

    Abstract: Objective: To construct a recombinant plasmid for use in RNA interference experiments on HnRNPA2/B1 and to verify its construction with sequence analysis. Methods: The 21bP HnRNPA2/B1 target sequence and the 7bP inverted repeat in the middle of the sequence were inserted in the PsiRNA-hH1neoG2 plasmid to produce the recombinant plasmid PSiRNA-hH1neoG2 HnRNPA2/ B1. Results: Sequencing analysis was performed on the recombinant plasmid. Conclusion: Successful construction of this vector may help to find a newtreatment method for various tumors.

     

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