Abstract:
Objective: To study the efficiency of silencing survivin gene expression level of survivin small RNA expression plasmid and its impact on the apoptosis, proliferation and invasive ability of colorectal cancer cell line SW480. Methods: The expression plasmid of survivin siRNA (pRNAT/ sur-siRNA) was constructed and transduced into SW480 cell line, was measured by the Western blot analysis and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) were used to measure the change in expression level of the survivin mRNA and protein, after transduction of the expression plasmid into the SW480. The apoptosis, proliferation, and invasive ability of SW480 cell line was evaluated by flow cytometry, MTT assay and Boyden Chamber Assay, respectively. Results: The expression plasmid (pRNAT/sur-siRNA) against survivin siRNA downregulated the survivin expression level of protein and mRNA dramatically in SW480 cell line, with a down-regulation of 85% and 80%, respectively. After transfection of the pRNAT/sur-siRNA in SW480, the apoptosis rate of pRNAT/sursiRNA/SW480 was16.9%, proliferation of pRNAT/sur-siRNA/SW480 cell was 37.4% and there was a remarkable difference comparing with the controls (P<0.01). Cell invasive experiment (Boyden Chamber Assay) showed that cell penetrating membrane number of the pRNAT/sur-siRNA/SW480, pRNAT/ SW480 and the SW480 cell was 153±66, 505±65 and 578±98, respectively (P<0.01). Conclusion: The siRNA against survivin can significantly inhibit the expression level of the survivin, thus promote apoptosis of SW480 cell and inhibit proliferation and invasive ability of the SW480 cell.