Abstract: Objective: To explore the significance for detection of IgH gene rearrangement in diagnosis of non-Hodgkin lymphoma. Methods: The heminested and multiplex polymerase chain reaction (PCR), in combination with family-specific primer mixtures (VH1, VH2, VH3) for IgH FR3A, FR2A, FR1 gene, were used to detect IgH gene cloning rearrangement in 44 patients with B-NHL, 1 with undifferentiated lymphoma, 15 with T-NHL and 5 with lymph node reactive hyperplasia. Results: a) In 44 cases with B-NHL, IgH gene rearrangement was found in 37 and detection rate was 84% using FR3A primer determination; IgH gene rearrangement occurred in 27 with B-NHL and the detection rate was 61% as using FR2A primer determination; IgH gene rearrangement was found in 34 and 33 cases respectively when using the FR1 family specific primer Mixtures (VH1, VH2 and VH3) and J area (JHb, JHc) primer, and the detection rate was 77%, 75% respectively. The combination of FR3A, FR2A and FR1 family specific primers mixture (VH1, VH2 and VH3) was used, with a total detection rate of 95%. b) In 15 cases with T-NHL, IgH gene rearrangement was found in 4 and was not found in 5 with LRH. c) One case with indiscriminate immunohistochemical NHL was defined as B-cell lymphoma after PCR determination. Conclusions: Application of multiple pairs of primers can enhance positive rate of the test and the determination of IgH gene rearrangement will be of important significance for differentiation of B-cell lymphoma and lymph-node reactive hyperplasia, as well as T-cell lymphoma.