王金环, 刘家美, 卢轶, 徐新女, 王淑杰, 郭承承, 刘宏胜, 张益伟, 白雪. LGI1基因对胶质瘤细胞作用的研究[J]. 中国肿瘤临床, 2006, 33(21): 1217-1220.
引用本文: 王金环, 刘家美, 卢轶, 徐新女, 王淑杰, 郭承承, 刘宏胜, 张益伟, 白雪. LGI1基因对胶质瘤细胞作用的研究[J]. 中国肿瘤临床, 2006, 33(21): 1217-1220.
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Wang Jin Huan, Liu Jia Mei, Lu Yi, et al, . The role of leucine-rich glioma inactivated gene-1 on the glioma cell[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2006, 33(21): 1217-1220.
Citation: Wang Jin Huan, Liu Jia Mei, Lu Yi, et al, . The role of leucine-rich glioma inactivated gene-1 on the glioma cell[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2006, 33(21): 1217-1220.
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LGI1基因对胶质瘤细胞作用的研究

The role of leucine-rich glioma inactivated gene-1 on the glioma cell

    摘要
  • 摘要: 目的:研究富亮氨酸胶质瘤失活基因1(LGI1)与胶质瘤细胞生长及胶质瘤细胞凋亡的关系。方法:构建pcDNA3.1/LGI1质粒,采用脂质体介导的方法转染LGI1缺失的胶质瘤细胞系。RT-PCR法和免疫组化法检测转染细胞的LGI1基因表达,MTT法检测转染细胞的增殖活性,AnnevinV-FITC法检测转染细胞的凋亡。结果:pcDNA3.1/LGI1转染胶质瘤细胞系A172后,A172细胞LGI1mRNA和蛋白表达阳性;细胞增殖活性受到抑制,MTT吸收值在转染后24和48小时下降,与正常对照组相比有统计学意义(P<0.05);转染后24小时早期凋亡细胞较多,48小时后早期凋亡减少。结论:LGI1基因可抑制肿瘤细胞的增殖,诱导细胞凋亡。

     

    Abstract: Objective: To research the relationship between LGI1 and glioma cell proliferation and apoptosis. Methods: pcDNA3.1/LGI1 plasmid was constructed and transfected into A172 glioma cell which was found without LGI1gene expression mediated by lipofectamine. The expressions of LGI1 mRNA and protein in the positive clones were detected by RT-PCR and immunohistochemistry and the proliferating activity and apoptosis of the cell were detected by methods of MTT and Annexin V-FITC. Results: After A172 cell was transfected by pcDNA3.1/LGI1, the expressions of LGI1 mRNA and protein were positive significantly. The proliferating activity of the cell was inhibited. The MTT OD value was reduced at 24 hour and 48 hour (P<0.05, compared with normal control group). Early apoptic cells at 24 hour were much more than that at 48 hour. Conclusion: LGI1 may inhibit cell proliferation and induce the cell apoptosis.

     

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