Abstract: Objective: To investigate the inhibition of c-myc antisense oligodeoxynucleotides mediated by receptor on the growth of mice transplanted hepatocarcinoma. Methods: C-myc ASODN was mixed with galactose (Gal)- polyethyleneimine (PEI) reagent forming Gal-PEI-ASODN complex. The morphology of fluorescence-labeled Gal-PEI-ASODN entering Bel-7402 cells was observed under fluorescence microscope. Cell clones were counted by inverted microscope and the clone forming rates were calculated. Mice transplanted hepatocarcinoma model of Hep A cells was setting up, tumor inhibition rates were measured and C-myc protein expression level was examined by immunohistochemical method. Results: Gal-PEI-ASODN complexes could be imbibed by Bel-7402 cells effectively, fluorescence-labeled ASODN particles distributed on cell membrane surface, in cytoplasm or in nucleus. GalPEI-ASODN complexes inhibited cell clone forming significantly compared with the cell control group (P<0.01). Gal-PEI-ASODN complexes inhibited the growth of mice hepatocarcinoma with the tumor inhibition rate 40.25% (P<0.05) and blocked the expression of C-myc protein in hepatocarcinoma cells. Conclusions: C-myc ASODN mediated by galactose receptor can effectively inhibit the proliferation of Bel-7402 cells and the growth of transplanted hepatocellular carcinoma and the expression of C-myc protein in hepatocarcinoma cells in mice.