Abstract: Objective: To investigate protein expression in the molecular subtypes of diffuse large B-cell lymphoma (DLBCL), so as to provide discernible markers for the pathological diagnosis, treatment and prognosis of DLBCL. Methods: H&E and immunohistochemical staining were performed on samples from 52 cases with DLBCL. On the basis of immunohistochemical staining, the cases of DLBCL were further subtyped molecularly into the germinal center B-cell-like (GCB) and non-germinal center B-cell-like (non-GCB) groups, using CD10, MUM1 and bcl-6. Then the ratio of each subtype within DLBCL was analyzed in conjunction with the expression of CD10, MUM1 and bcl-6. Results: In this study, the number of non-GCB DLBCL cases was higher than the number of GCB DLBCL cases (69.2% versus 30.8%). Statistical analysis revealed a significant difference among the expression rates of CD10 alone, bcl-6 alone and CD10 and bcl-6 together in the GCB groups, in which the expression rate of CD10 ranked first, followed by bcl-6 and then CD10 and bcl-6 together. There was also a significant difference between the expression rates of MUM1 and MUM1 and bcl-6 together in the non-GCB groups. Conclusion: In China, most DLBCL cases originate from post-germinal center B-cells. The non-germinal center B-cell subtype is more prevalent and has a poorer prognosis. There was a significant difference in the expression of CD10, MUM1 and bcl-6 between the GCB and non-GCB groups that may be used for discrimination of the two subtypes.