FLIP基因在乳腺癌组织中表达的初步研究

Preliminary Studies on Expression of the FLIP Gene in Breast Cancer

摘要: 目的:探讨FLIP基因(包括蛋白和mRNA水平)在乳腺癌组织中的表达及对乳腺癌生物学行为的影响。方法:采用免疫组化和荧光实时定量PCR技术,检测乳腺浸润性导管癌和正常乳腺组织中FLIP蛋白及mRNA的表达。结果:FLIP_L/S蛋白在浸润性导管癌中的平均染色积分为6.13±0.34,在正常乳腺组织中的平均染色积分为2.68±0.30,两组有显著性差异(P<0.05)。FLIP_L、FLIP_S mRNA在浸润性导管癌中的含量均高于正常乳腺组织(P<0.05)。结论:FLIP在乳腺癌组织中高表达,可能通过阻断凋亡通路参与乳腺癌的发生。

Abstract: Objective: To investigate the relationship between expression of the FLIP gene and breast cancer. Methods: The expression of FLIP_L/S protein was detected using immunohistochemistry, and the FLIP_L/S mRNA was assayed by real-time fluorescent quantitative PCR. Results: The expression of FLIP_L/S protein in breast cancer (6.13±0.34) % was higher than in normal breast tissue (2.68±0.30)%. There was a significant difference between the two (P<0.05). The level of FLIP_L/S mRNA was significantly higher in breast cancer than in normal breast tissue (P<0.05). Conclusion: The FLIP gene is abnormally upregulated in breast neoplasm, hence it might play a crucial role in the carcinogenesis of breast cancer by blocking the receptor-mediated apoptotic pathway.