Abstract: To explore the anti- proliferative effects of anti- cancer Chinese medicinal materials such as aromatic turmeric oil on hepatoma (SMMC7721) and gastric carcinoma cells (NKM45). Methods: Bioluminescence assay (BLA) was applied to determine the ATP concentration when SMMC7721 and NKM45 were exposed to aromatic turmeric oil, cinobufacin and solanine. The effect of aromatic turmeric oil on the growth curve of NKM45 was also analyzed. Results: The concentration of ATP was significantly decreased when the tumor cells were exposed to 5- Fluorouracil(5 - FU). It was also was significantly decreased when SMMC7721 and NKM45 were treated with cinobufacin, solanine, or aromatic turmeric oil P<0.05~P<0.01). There was no significant difference in response between the tumor cells that were treated with the Chinese medicinal materials and those exposed to 5- FU (P>0.05). The aromatic turmeric oil significantly inhibited the survival rate of NKM45.Conclusion: Aromatic turmeric oil, cinobufacin, and solanine can inhibit the proliferative activity of SMMC7721 and NKM45. BLA assay can be used to screen anti- tumor drugs.